| Summary: | 碩士 === 國立交通大學 === 奈米科技研究所 === 96 === Recently, the microfluidic technology has become very important and widely used in many research fields such as biochemical technology, semiconductor technology and electronics technology. The microfluidic technology, the studies on the motion of fluid and particles through the microchannels, is an emerging field that has given rise to a large number of scientific and technological developments over the last years.
In this study, we would like to develop a microfluidic reactor for production of the biodiesel of transesterification reaction. First, we immobilized lipase on the tetramethyl ammonium hydroxide (TMAH) textured surface with self-assembled monolayer (SAMs) of covalent bonding. The TMAH textured surface for anisotropic texturisation are usually growing pyramidal structures which are increasing the surface areas for lipase-immobilized and decreasing the reflectivity for advancing light absorption. The immobilization of lipase on solid substrate is an essential step for many applications in the field of biocatalysis duo to the relevance for the performance to improve and optimize the lipase activity and stability. However, the transesterification by enzyme method is time-consuming compared to acid- or alkali-catalyzed. Development the microfluidic reactor system is an important way to improving the reaction rate and enhancing the conversion yield due to the high surface-volume ratio and advancing the mass and heat transfer. This experiment aims is to provide a new approach that could potentially analyze the real-time reaction of transesterification. Moreover, new generation of biosensors combining new bioreceptors with the ever-growing number of transducers is emerging.
The microfluidic reactor transducers of analysis systems are used in the optical spectroscopy (UV-Vis spectroscopy) for detecting the transmission of transesterification reaction. Following is detecting by nuclear magnetic resonance (NMR) for identifying the structure of biodiesel and then estimating the conversion of reaction. Since the response of transmission can be detected from transesterification reaction, the exploration of photo-electronic analysis, photodetector, can be used for monitoring the biocatalytic reaction in real-time. It is based on the phenomenon of different transmission and converted to electric signal. Since the detection of UV/Vis-NMR can confirm the conversion of transesterification and the detection of optical-electric (UV/Vis-photodetector) can be real-time monitoring the transesterification reaction. Combine with above analysis methods, the experiments provide a new approach for an easy and feasible way to analyze and detect the transesterification reaction.
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