An optical bio-sensing system for the determination of glucose and histamine based on CMOS photodiode chip

• Main Authors: Ping-chun Yu, 游秉鈞
• Other Authors: Yuh-Shyong Yang
• Format: Others
• Language: en_US
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/06283494578998876453

碩士 === 國立交通大學 === 理學院碩士在職專班應用科技學程 === 96 === We developed a biomedical diagnosis system by integrating CMOS photodiode chip and coupled enzyme reactions. Glucose and histamine are the chosen targets because of their importance in the medical diagnosis. The Km value of glucose and histamine obtained from our assembled system is 4.9 and 125 uM, respectively, which are similar to those obtained from UV/Vis spectrophotometer (7.2 and 160 uM). The detection limits of glucose and histamine concentrations in our system are 1 uM and 10 uM, respectively. The linear ranges are from 1 uM to 5 mM and 10 to 80 uM for glucose and histamine, respectively, which both are 2 to 10 folds lower than those in the human blood. In the future, our research will particularly aim at multi-functional household medical diagnosis instrument.