HOS Cyto-compatibility of Titanium Alloy Coated with Ca-P Compound and Collagen Films

• Main Authors: Po-kai Chuang, 莊博凱
• Other Authors: Tze Ming Lee
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/77201402571179607056

碩士 === 國立成功大學 === 製造工程研究所碩博士班 === 96 === Surface modification of the substrate affects it’s cyto-compatibility. We have evaluated the HOS cytocompatibility of Ti-6Al-4V alloy after coated with Ca-P compound, type I collagen, and compound coating of both on the surface respectively. Experiment was divided into four groups, called Ti-6Al-4V, Ca-P, Collagen, Ca-P/Collagen, respectively. Before surface modification, surface of Ti-6Al-4V alloy became smooth after abrading with sandpapers and polishing with Al2O3. For Ti-6Al-4V group, sample surface coated with nothing. For Ca-P group, sample surface coated with Ca-P compound by biomimetic immersion ( simulated body fluid ; SBF ). For Collagen group, sample surface coated with type I collagen ( from calf skin ). For Ca-P/Collagen group, sample surface coated with Ca-P compound and type I collagen. After human osteosarcoma ( HOS ) cells were cultured in each groups, we evaluated cell proliferation and observed cell morphology. When cell metabolism was more active, more Methylthiazoletetrazolium ( MTT ) was wasted and more formazan crystal was appeared. After cultured for 1, 5 and 10 days, we assayed proliferation of HOS cells by ELISA reader. Cells were cultured for 1, 3, 12 and 24 hours, we observed cell morphology on sample surface by scanning electron microscopy ( SEM ). Results of experiment, we found that type I collagen was the best factor and Ca-P compound was the worst factor to enhance cell proliferation. For cell morphology, type I collagen could help to flatten HOS cells sample surface.