| Summary: | 碩士 === 國立成功大學 === 藥理學研究所 === 96 === Sepsis is the systemic inflammatory response to infection. In spite of marked advance in medicine, the mortality rates in septic patients are still rising. Sepsis can cause failures of multiple organs, including heart, lung, liver, kidney and gastrointestinal system. Global tissue hypoxia results in an imbalance between systemic oxygen delivery and demand, and is a key development of preceding multiple organ failure and death. Nevertheless, the mechanisms of gastric hemorrhagic ulcer in sepsis are unknown. Taurine is one of the most abundant amino acids in the body. Melatonin is secreted by the pineal gland. Both taurine and melatonin possess potential antioxidant effects. Whether these two drugs can ameliorate gastric hemorrhagic ulcer in septic rats is unknown. Intraperitoneal lipopolysaccharide (LPS; 10 mg/kg) was challenged to rats for 24 hours to induce sepsis. Animals were divided into four groups: (1) Sham operation (Sham) (2) Sham + melatonin (10、20、50 mg/kg); Sham + taurine (50、100、250 mg/kg) (3) LPS (4) LPS + melatonin; LPS + taurine. After gastric surgery, the rat stomachs were then irrigated for three hours with simulated gastric juice, and then to evaluate effects of melatonin and taurine on LPS-induced septic rats. At 0 and 24 hr after LPS treatment, the changes of white blood cell count, blood sugar, rectal temperature, gastric ulcerogenic factors (gastric acid back diffusion, histamine concentration, lipid peroxidation), gastric defensive parameters (gastric mucus and glutathione production in gastric mucosa), antioxidant effects (catalase, superoxide dismutase, glutathione reductase and glutathione peroxidase) were examined. The results showed that LPS produced severe gastric hemorrhagic ulcer and exacerbation of offensive factors while defensive parameters were decreased. These beneficial effects of melatonin and taurine may be attributed to reducing organ O2- levels and increasing activation of antioxidant enzymes (catalase, superoxide dismutase, glutathione reductase and glutathione peroxidase), thereby decreasing the mortality rates in LPS-induced septic rats.
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