A Study of Hydrodynamic Focusing Micro-Channel Flow and the Control of its Flow Rate Distribution for Micro Particle Separation

• Main Authors: Kai-Cheng Wang, 王凱正
• Other Authors: Denz Lee
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/43141085556906202575

碩士 === 國立成功大學 === 航空太空工程學系碩博士班 === 96 === Micro-Electro-Mechanical-System（MEMS）is developed rapidly in recent years, and it facilitates the study of micro total analysis system（μ-TAS）. The system offers several potential advantages. It needs very small volume of samples and reagents, produces little waste, and offers short reaction and analysis time. It can decrease the cost by combing with micro-fabrication technology and has various applications for cell study、cell separation and drug detection. In this study, we use numerical analysis to construct microchannel simulation system for multi-particle separation. The channel is designed and assessed according to flow resistance theory. This research utilizes hydrodynamic focusing and asymmetrical exit channel resistance design to enhance particle separation. We can predict particle outlet more accurately by controling the flow distribution in pinch segment. In this study, fabrication is based on PDMS elastomer. The master is formed on silicon wafer using an epoxy-based photoresist. PDMS is cast against the master to produce molded layer containing channels which is then bonded on a glass to form the chip. The result of the experiments and numerical simulation are compared and discussed. The particles of 2 μm、6 μm and 10 μm in diameter can be separated in our microchannel design through their different displacement paths.