Effect of poly IC and ribavirin against enterovirus 71 infection in human neural cells

• Main Authors: Han-Hsuan Chung, 鍾瀚璿
• Other Authors: Trai-Ming Yeh
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/23258391204891462331

碩士 === 國立成功大學 === 醫學檢驗生物技術學系碩博士班 === 96 === Enterovirus 71 (EV71) is the main causative agent of hand-foot-mouth disease, herpangina and has been associated with severe neurological disease like meningitis, encephalitis resulting in high mortalities. There is no effective antiviral drug could be used to cure the infection of EV71. In this study, we choose the interferon inducer poly IC and ribavirin to evaluate their effects on EV71 infection of human neuron cells. First, we selected two neural cell lines, SK-N-SH and U118MG to set up an in vitro EV71 infection model. We found EV71 infection in both cell lines induced the formation of cytopathic effect. EV71 antigen was also detected in the cytoplasm of EV71-infected cells. In addition, inflammatory cytokines such as macrophage migration inhibitory factor and interleukin-8 were increased in EV71-infected cells in a dose- and time-dependent manner. Treatment of poly IC could reduce the viral titer and inflammatory cytokines and increase the cell viability in SK-N-SH but not in U118MG cells. Ribavirin, on the other hand, possesses antiviral activity in both cell lines against EV71 infection. However, higher cytotoxicity of ribavirin in SK-N-SH was observed. Nevertheless, Synergenic effect of the combination usage of poly IC and ribavirin against EV71 infection of neural cells was found. Therefore, the cytotoxicity of ribavirin could be reduced by the combination usage of poly-IC. These results suggest the combination usage of interferon or its inducer and ribavirin in EV71 infection may worth to be used in patients.