| Summary: | 碩士 === 國立成功大學 === 工程科學系碩博士班 === 96 === The resonance condition of surface plasmon resonance (SPR) is easily destroyed by environmental disturbances on the interface between metal and dielectric medium. Therefore, with the helps of high sensitivity about the disturbances, biomolecular interaction on the interface can be quantitatively determined. Additionally, there are some other advantages such as real-time analysis, label free, kinetics study, and high-throughput screening, the SPR biosensors play a significant role in biomolecular interaction analysis (BIA).
Conventional SPR biosensors, however, detect the overall signal on the sensing surface according to specific biomolecular interactions, nonspecific interactions (e.g. biomolecules adsorption on the sensing surface), and background interferences (e.g. the refractive index change and fluctuation of buffer solution, temperature fluctuation), so interested specific interaction signal is difficult to be exactly extracted from the detection signal. Therefore, this study has developed a new SPR instrument with dual laser beams based on attenuated-total-reflection (ATR) method. Also, its sensor chip has two sensing regions corresponding to the dual laser beams: one with surface modified bio-receptors, and the other without any bio-coating. The dual-laser-beam SPR system not only retains original advantages of conventional SPR, but also discriminates the specific response from the nonspecific interaction and background interferences. Certainly, the SPR system will be beneficial for real-time BIA.
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