Study of Drug Induced Colitis and Colorectal Cancer in Mice

• Main Authors: Wen-Di Wu, 吳聞笛
• Other Authors: Chia-hung Mao
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/76974809586924687817

碩士 === 國立中興大學 === 獸醫學系暨研究所 === 96 === The severities of colonic inflammation were assessed 3 and 14 days after rectum injection of 2,4-Dinitrobenzene sulfonic acid (DNBS) 2 or 3 mg in C57BL/6JNarl male mice. The pathological results showed DNBS produced acute colitis 3 days after first administration with inflammatory cells aggregation and mucosa necrosis, especially in certain sections of colon. However, on day 14, the inflammation had subsided and the mucosa was recovered to normal. Therefore, this animal model is suitable for the study of acute colitis than chronic colitis or further colorectal cancer research. Previous studies showed that injection of azoxymethane (AOM) alone or with oral exposure to dextran sodium sulfate (DSS) could induce mouse colorectal cancer. In this study, C57BL/6JNarl and BKS.Cg-+Leprdb/+Leprdb male mice were treated subcutaneous with AOM 10 mg/kg once a week for 3 weeks. The aberrant crypt foci (ACF) can only be observed after 40 weeks. On the other hand, BALB/cByJNarl mice were given a single intraperitoneal injection of AOM, followed by 1% DSS in drinking water for 7 days, ACF can be observed after 22 weeks. These results indicated that drugs and mouse strains coordination should be considered for ACF induction in the establishment of intestinal disease mouse model.