The immunophenotype and prognosis in dogs with B-cell or T-cell lymphomas

• Main Authors: Chiao-Yun Lin, 林巧雲
• Other Authors: Shih-Chieh Chang
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/50229533011462165507

碩士 === 國立中興大學 === 獸醫學系暨研究所 === 96 === Abstract Lymphoma is the most common hematological malignancy. It can be classified as T-cell and B-cell lymphomas based on its origin. The etiology remains obscure. The ranges of survival times and the response to chemotherapy vary among individuals. The immunophenotype and morphology subtype of lymphoma was suggested according to previously research. The aim of this study is to classify the phenotype and morphology associated with prognosis in canine lymphomas. 35 cases of canine lymphoid malignances, including 25 multicentric lymphomas, 4 cutaneous lymphomas, 1 sus-pected intestinal lymphoma, 1 extranodual multicentric lymphoma and 4 leukemias, were collected from our hospital during the period from 2004 to 2008l. Diagnosis was confirmed by the combination of clinical, cytological, and histological examinations. Morphological determination was based on the Working formulation (WF) and the up-date Kiel morphologic classification systems. Immunohistochemistry (IHC) and poly-merase chain reaction (PCR) methods were utilized to classify the phenotype of lym-phomas. Among the patient, mixed breed was the most common breed with lymphoma in this study and B-cell subtype (60%) was predominant in multicentric lymphomas. Intermediate grade (70%) was the most frequent type of lymphomas. In this study, PCR method was successfully applied in immunophenotyping and monitoring the efficacy of treatment of dogs with lymphomas or leukemia. Mean overall survival time in treated dogs and untreated dogs with lymphomas were 177 days (ranging from 14 to 741 days) and 19 days (ranging from 15 to 137 days), respectively. No significant relationships were found between sex, age and survival time. According to Cox proportional hazards model method, the response of chemotherapy and clinical stage were the main factors influencing one-year survival time in dogs with malignant lymphomas.