| Summary: | 碩士 === 國立中興大學 === 生物產業機電工程學系所 === 96 === In the study, we fabricated a conductimetry-integrated capillary electrophoresis chip with 3D pair-electrodes. The chip was made by using photo lithography techniques on ITO glasses and the negative photo resist (SU8-3010) was used for forming the microchannel walls. Following the hot bonding at 90℃with 20 kgf for 2 min, the successful ratio of the chip bonded was 90% and the deformation of channel width was 4.76%. Moreover, the decoupler electrode was electrodeposited with palladium material to adsorb the hydrogen. The experimental results showed that the decoupler with 5 min electrodeposition of palladium can prevent the formation of hydrogen bubbles under the electric field of 100 V/cm. The cell constant of 3D pair-electrode calculated is 12.48/cm. Present, the detection concentration was 0.5 mM K+ (S/N:75) and 0.5 mM Na+(S/N:17) in 5 mM MES/His running buffer, respectively. We can adjust the composition of running buffer so as to obtain the lower detection limit and the better sensitivity for the detection of lower concentration samples.
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