The culture and application of orchid mycorrhizal fungi to promote seed germination and the seedling growth in Cattleya.

• Main Authors: Yu-Jui Chuang, 莊育瑞
• Other Authors: Tsai-Yih Wang
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/03176210147441075571

碩士 === 國立中興大學 === 園藝學系所 === 96 === Studies on orchid seed symbiotic culture indicated that inoculate Cattleya orchid seeds with different orchid mycorrhizal fungi (OMF) resulted in different level of seed development promotion. Inoculate orchid seeds with OMF at the 0 day and the 4th day after germination both increased the seed germination. In photoperiodic treatment of OMF inoculated orchid seeds, 24 hours light period with TF fungi treated could gain higher level germination rate; where 24 hours dark period with PT fungi treated could also promote the orchid seed development. OMF suspension liquid will either enhance orchid seed germination. Studies on Cattleya [(Brassavola nodosa × Cattleya aurantiaca)× Sophronitis cernua] off bottle seedling with OMF inoculated indicated that increased plant characters on plant fresh weight, plant dry weight, plant height, leaf length, leaf width, and the length, width and number of both root and pseudobulb. In different culture media effect on OMF inoculated orchid seeding, using sphaghnum moss with TF strain treated in planting off bottle seedlings resulted in increased plant fresh weight, plant dry weight, plant height, leaf length, leaf width, and the length, width and number of both root and pseudobulb. TF strain treated on off bottle orchid seedlings with best result performed in 3 roots and above per seedling and the least in seedling without roots where the survival rate was 90%. When fertilize the orchid seedlings with OMF added once two and four weeks will also significantly enhanced plant fresh weight, plant dry weight, plant height, leaf length, leaf width, and the length, width and number of both root and pseudobulb. In carbon utilization, TSa and TL strains used glucose; PT and TF strain use sucrose as its carbon source while replication in order to produce high amount of mycelium. In nitrogen utilization, the best was in yeast extract usage and the least were in (NH4)2SO4 and NH4NO3. In culture method effects on mycelium production mainly depend on OMF growth feature. Growth of Rhizoctonia spp. will inhibit by light, therefore, dark culture could decreased the mycelium production period and could obtain larger mycelium amount in the same time as in light culture. In different culture media effect on Rhizoctonia spp. mass production experiment, the results showed that TL, TF, and TSa cultivate with GYM media and PT strain cultivate with CM media could obtained best result. The growth curved of Rhizoctonia spp. is similar, mycelium amount increased as culture period lengthen. After reaching the peak at the 12th days and the 18th day, the mycelium growth slow down and decreased at the 30th day significantly.