Study on Characteristics, Germination, Vigor Test, and Coating Technique of Cabbage (Brassica oleracea L. var. capitata L.) Seed.
碩士 === 國立中興大學 === 園藝學系所 === 96 === Cabbage seeds are small, smooth, brown, and globular. The seeds of three cabbage cultivars were studied the relationship between seed components and radicle protrusion time. The most appropriate method of assessing seed vigor and the various presowing treatments on...
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ndltd-TW-096NCHU53780012016-05-11T04:16:23Z http://ndltd.ncl.edu.tw/handle/56630330852483282322 Study on Characteristics, Germination, Vigor Test, and Coating Technique of Cabbage (Brassica oleracea L. var. capitata L.) Seed. 甘藍種子特性,發芽,活力檢測與披衣技術之研究 Nan Lar (a) Nan Kyi Yin 汪芝穎 碩士 國立中興大學 園藝學系所 96 Cabbage seeds are small, smooth, brown, and globular. The seeds of three cabbage cultivars were studied the relationship between seed components and radicle protrusion time. The most appropriate method of assessing seed vigor and the various presowing treatments on the germination of seeds of ‘Autumn King’ were evaluated. Scanning electron microscopy revealed that the outer seed coats of all three cabbage cultivars and the inner seed coats of ‘228’ and ‘Autumn King’ were of the reticulate–foveate type, while the inner seed coat of ‘CA49’ was of the reticulate type. Seeds of the three varieties had different germination periods, with the radicle protrusion time for ‘CA49’, ‘228’, and ‘Autumn King’ being approximately 11, 17, and 25 h, respectively. Seed of ‘228’ absorbed the least water. Among the three cultivars, ‘CA49’ seed contained the highest levels of total soluble sugar and crude fat, ‘228’ contained the highest levels of starch and soluble protein, and ‘Autumn King’ contained the highest level of sinapine. Vigor tests carried out on ‘Autumn King’ cabbage seed reveled that germination performance decreased significantly with increasing aging time. The electrical conductivity of the seed leachate and the levels of soluble carbohydrate, free amino acid, and potassium ion in the leachate from aged seeds were about double that of control seeds. The control seed produced carbon dioxide at a rate significantly higher than that of seed aged 36 h and 48 h 1.2– and 2.7–fold, respectively. The dehydrogenase activity of control seed was significantly higher than that of aged seed. In the tetrazolium test, the completely and the unevenly stained seeds demonstrated a high level of vigor, and these seeds were present at great proportions of the control seed. Sinapine leachate could be used in detecting seed quality among seed lots. The sinapine fluorescent of control and primed seeds was present under UV, while seeds were imbibed for 4–24 h and 1–3 h, respectively. The emergence rate was highly correlated with the values of the vigor tests. At 35°C, the germination percentage of control ‘Autumn King’ seeds, 73.3%, was significantly lower than that of the primed seeds or soaked seeds, with or without a coating, with the germination percentage of the latter ranging from 88.3% to 95.0%. The emergence and normal seedling percentages of seeds subjected to the priming–coating with 0.3% methyl cellulose treatment were significantly higher than those of the control seeds or seeds coated with 0.4% arabic gum. The sinapine leakage index (SLI) and the fluorescent percentage of ungerminated seeds of the priming–soaking– sorting–coating treatments were zero. Following storage for 6 months, the emergence percentage of control, primed, and soaked seeds without coating significantly decreased by more than 3%. The number of mean days to emergence for seeds of the storage seed lot was significantly longer than 4 days. After accelerated aging for 36 h, the germination percentage was significantly lower only in primed seed (8%) compared to primed or soaked seeds coated with 0.3% methyl cellulose. These results indicated that cabbage seed could be treated by priming–soaking–coating to maintain germination quality. Yu Sung 宋 妤 2007 學位論文 ; thesis 81 en_US |
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碩士 === 國立中興大學 === 園藝學系所 === 96 === Cabbage seeds are small, smooth, brown, and globular. The seeds of three cabbage cultivars were studied the relationship between seed components and radicle protrusion time. The most appropriate method of assessing seed vigor and the various presowing treatments on the germination of seeds of ‘Autumn King’ were evaluated.
Scanning electron microscopy revealed that the outer seed coats of all three cabbage cultivars and the inner seed coats of ‘228’ and ‘Autumn King’ were of the reticulate–foveate type, while the inner seed coat of ‘CA49’ was of the reticulate type. Seeds of the three varieties had different germination periods, with the radicle protrusion time for ‘CA49’, ‘228’, and ‘Autumn King’ being approximately 11, 17, and 25 h, respectively. Seed of ‘228’ absorbed the least water. Among the three cultivars, ‘CA49’ seed contained the highest levels of total soluble sugar and crude fat, ‘228’ contained the highest levels of starch and soluble protein, and ‘Autumn King’ contained the highest level of sinapine.
Vigor tests carried out on ‘Autumn King’ cabbage seed reveled that germination performance decreased significantly with increasing aging time. The electrical conductivity of the seed leachate and the levels of soluble carbohydrate, free amino acid, and potassium ion in the leachate from aged seeds were about double that of control seeds. The control seed produced carbon dioxide at a rate significantly higher than that of seed aged 36 h and 48 h 1.2– and 2.7–fold, respectively. The dehydrogenase activity of control seed was significantly higher than that of aged seed. In the tetrazolium test, the completely and the unevenly stained seeds demonstrated a high level of vigor, and these seeds were present at great proportions of the control seed. Sinapine leachate could be used in detecting seed quality among seed lots. The sinapine fluorescent of control and primed seeds was present under UV, while seeds were imbibed for 4–24 h and 1–3 h, respectively. The emergence rate was highly correlated with the values of the vigor tests.
At 35°C, the germination percentage of control ‘Autumn King’ seeds, 73.3%, was significantly lower than that of the primed seeds or soaked seeds, with or without a coating, with the germination percentage of the latter ranging from 88.3% to 95.0%. The emergence and normal seedling percentages of seeds subjected to the priming–coating with 0.3% methyl cellulose treatment were significantly higher than those of the control seeds or seeds coated with 0.4% arabic gum. The sinapine leakage index (SLI) and the fluorescent percentage of ungerminated seeds of the priming–soaking– sorting–coating treatments were zero. Following storage for 6 months, the emergence percentage of control, primed, and soaked seeds without coating significantly decreased by more than 3%. The number of mean days to emergence for seeds of the storage seed lot was significantly longer than 4 days. After accelerated aging for 36 h, the germination percentage was significantly lower only in primed seed (8%) compared to primed or soaked seeds coated with 0.3% methyl cellulose. These results indicated that cabbage seed could be treated by priming–soaking–coating to maintain germination quality.
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author2 |
Yu Sung |
author_facet |
Yu Sung Nan Lar (a) Nan Kyi Yin 汪芝穎 |
author |
Nan Lar (a) Nan Kyi Yin 汪芝穎 |
spellingShingle |
Nan Lar (a) Nan Kyi Yin 汪芝穎 Study on Characteristics, Germination, Vigor Test, and Coating Technique of Cabbage (Brassica oleracea L. var. capitata L.) Seed. |
author_sort |
Nan Lar (a) Nan Kyi Yin |
title |
Study on Characteristics, Germination, Vigor Test, and Coating Technique of Cabbage (Brassica oleracea L. var. capitata L.) Seed. |
title_short |
Study on Characteristics, Germination, Vigor Test, and Coating Technique of Cabbage (Brassica oleracea L. var. capitata L.) Seed. |
title_full |
Study on Characteristics, Germination, Vigor Test, and Coating Technique of Cabbage (Brassica oleracea L. var. capitata L.) Seed. |
title_fullStr |
Study on Characteristics, Germination, Vigor Test, and Coating Technique of Cabbage (Brassica oleracea L. var. capitata L.) Seed. |
title_full_unstemmed |
Study on Characteristics, Germination, Vigor Test, and Coating Technique of Cabbage (Brassica oleracea L. var. capitata L.) Seed. |
title_sort |
study on characteristics, germination, vigor test, and coating technique of cabbage (brassica oleracea l. var. capitata l.) seed. |
publishDate |
2007 |
url |
http://ndltd.ncl.edu.tw/handle/56630330852483282322 |
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