| Summary: | 碩士 === 國立中興大學 === 動物科學系所 === 96 === The purpose of this study was to investigate the in vitro fermentation patterns of different feedstuffs by automated pressure evaluation system (APES) using chicken fecal inocula, kinetic parameters, end-productions and dry matter loss(%) to evaluate the fermentation characteristics of feed ingredients effects in broiler. Eighteen feedstuffs included cereals (corn, barley, rice, wheat, and cassava), cereal byproducts (wheat bran, and wheat middling), processed feedstuffs (popping corn, extrusion corn, and roasted potato), protein sources (SBM, rapeseed meal, fermented soybean meal, corn gluten meal, fish meal, and meat bone meal,) and distiller’s dried grains soluble (DDGS) (corn and milo) were used to evaluate the fermentation characteristics. The results indicated the processed feedstuffs and rice were fermented more rapidly than cereals and cereal byproducts , the protein sources and DDGS are the least. The total gas volume, dry matter losses and SCFA showed similar trend in test samples. But the ammonia concentration trend was opposite. After in vitro digestion, the gas productions of all feedstuffs decreased except for cassava. The gas production of processed feedstuffs was lower than cereals and protein sources lower than without in vitro digestion. According to the in vitro results, we chose two feedstuffs with different fermentation rate, dehulled barley (fast) and wheat (slowly), and the corn diet as the control for in vivo feeding experiment. The data suggested the feed conversion ratio (feed / gain, FCR) of dehulled barley and corn diet were better than wheat diet (1.73, 1.70 verse 1.81). The wheat diet decreased the anaerobic bacteria count in caecal and aerobic bacteria count in colon at 21day. In ammonia concentration and pH value, the wheat diet was lowest. In conclusion, the APES is to go with kinetic parameters and end-productions analysis could predict in vitro fermentation patterns of different feedstuffs. Although in vitro gas fermentation were different between dehulled barley and wheat and the intestine condition accurately changed, the growth of broiler is involved in many factors. We are not sure that fermentation rate is a major factor in broiler growth. More experiments are necessary for confirmation of this hypothesis.
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