Association of Polymorphisms of Differentially Expressed Transcripts in Adipose Tissue of Taiwan Country Chickens with Performances

• Main Authors: Shu-Hsien Li, 李淑嫺
• Other Authors: 黃木秋
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/07448524908277790544

碩士 === 國立中興大學 === 動物科學系所 === 96 === The purpose of this study is to investigate the SNPs (single nucleotide polymorphisms) genotypes of differentially expressed genes in adipose tissue of Taiwan country chickens, and their correlations with laying and growth Traits. The SSCP (single strand conformation polymorphism) analysis of AFABP, apoVLDLII, CST3, CNOT8, FTH1, GOT1, GSN, ITFG1, PDIA3 and PDPK1 genes was performed. The different types of SSCP patterns in all these genes were found. Nucleotide sequencing was carried out. Twenty-one SNPs were observed in these 10 gene sequences. Genotyping of 219 L2 and 93 B lines of Taiwan country chickens, detected were carried by minisequencing. The different genotypes of these genes were presented in chickens. The correlations between genotypes and performances of chickens were investigated. The results showed that the age of first egg (AFE) of C332T-CC and G412A-GG in AFABP, are significantly earlier than TT and AA types (P < 0.05). While the T97G, T139G, A186G, T236C and T267C of CST3 gene are TT, TT, AA, TT and TT types respectively, the number of total eggs (Eggs) and hen day rate (HDR) are significantly higher than GG, GG, GG, CC and CC types (P < 0.05); Whereas, the mean pause day (MPD) of T97G-TT is significantly shorter than it of T97G-GG; Furthermore, when the T236C and T267C of CST3 are TC heterozygote, the mean lay day (MLD) and mean clutch egg (MCE) are significantly higher than that are CC genotype (P < 0.05); Body weight (BW) of T139G-TT and A186G-AA, T236C-TT/TC and T267C-TT/TC genotypes in CST3 are significantly higher than that of the other homozygotes. MLD, MCE and max clutch egg of G27A-AA type in GOT1 are significantly higher than those of GG and GA types (P < 0.05). The chickens with nt173-175 (－/－) and C219T-TT genotypes in GSN are significantly heavier than the chickens with nt173-175 (＋/＋) and C219T-CC genotypes for BW at 12-36 weeks (P < 0.05). Chickens with G78A-GA genotype of ITFG1 are significantly higher than those with GG type for max clutch egg (P < 0.05). Chickens with C268T-CC genotype of in PDPK1 are significantly shorter than those with TT type for AFE and MPD (P < 0.05). We conclude that C332T-CC and G412A-GG of AFABP; T97G-TT, T139G-TT and A186G-AA or allele T of T236C and T267C in CST3; G27A- AA of GOT1; G78A-GA of ITFG1; and C268T-CC of PDPK1, can be used in a selection program for develop of egg production. Furthermore, T139G-TT, A186G-AA and allele T of T236C and T267C in CST3; nt173-175 (－/－) and C219T-TT of GSN, acted in dominant fashion on growth performance of chickens. The results could be applied these genotype loci in the large scale chickens breeding improvement programs.