Studies on the Antioxidant Defense Activity and Functional Substances Induced during Short-Time Germinated Brown Rice by Two Sources of Light

• Main Authors: Wen-Meei Wang, 王文美
• Other Authors: 盧 訓
• Format: Others
• Online Access: http://ndltd.ncl.edu.tw/handle/19767996594584476922

博士 === 國立中興大學 === 食品暨應用生物科技學系 === 96 === Two brown rice varieties, Taichung Sen 10 (TCS10, indica) and Taikeng 8 (TK8, japonica) were used as germination tested samples. The antioxidative enzyme activities (AEA), like as superoxide dismutase, catalase, glutathione reductase, ascorbate peroxidase, dehydroascorbate reductase, and total peroxidase etc, and the level of the non-enzymatic antioxidants, glutathione (GSH) and ascorbic acid (ASC), as well as the total antioxidant activity (TAA), Malondialdehyde (MDA),Total peroxide and the functional substances of γ-aminobutyric acid (GABA), oryzanol and inositol were all examined and then compared between two varieties of germinated brown rice (GBR) by two sources of light in this study. The effect of re-drying processes will also be evaluated. Germination process of the GBR was conducted for 15, 18, or 21 hr duration period respectively under either normal light (NL) or far infrared rays (FIR, 8-12μm) lightening in a growth chamber, the temperature of growth chamber was controlled at 25℃±2℃. The slightly germinated brown rice (GBR) was then divided into three parts before stored in a -81℃ freezer for further uses : first one is wet germinated brown rice without further treatment; second one is freeze-dried and the third one is room temperature dried. The data showed that the FIR treatments, when comparing with NL treatments, indeed enhance the germination processing to occur earlier. The resulting data also showed that the short time germinated brown rice possess greater activities of AEA, levels of antioxidant and contents of functional substances than those ungerminated control. The ungerminated control and GBR from TCS10 variety had a higher AEA (except catalase and MDA), GSH and ASC levels than those of TK8 variety. Germinated TCS10 samples also had a higher total antioxidant ability (ABTS) and GABA contents than those did of control and germinated TK8 ones. FIR lightening treated samples promoted better AEA, GSH and ASC levels, TAA and GABA contents than those produced by germination under normal light treated. The data further revealed that the way of room temperature dried had higher antioxidative enzyme activities than those freeze-dried. Thus, Under the experiment conditions in this study, we concluded that short time germination process, especially lightening treated by FIR, could enhance biochemical activity and result in improving the nutritional and physiological benefits of brown rice. The effect from its wave length may greater than the promoting temperature one. Taichung Sen 10 (TCS10, indica) variety could be another nice chosen as healthy GBR material. Ascorbic acid should play an important role inside the ascorbate-glutathione cycle.