A Kinetic Parameter Study of a Thermophilic Aerobic System Treating High Strength Oily Wasterwaters

• Main Authors: Yu-Tzu Shen, 沈育資
• Other Authors: C-hih-Jen Lu
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/57477274581397555785

碩士 === 國立中興大學 === 環境工程學系所 === 96 === The research utilized the thermophilic wasted sludge colleeded from a food company in Guanyin Industrial Park as the micbacterial resource. After the acclionation with a lab-scale reactor for more than six months, obtaining the pure culture strain of bacteria was identified through the process of gene mapping by a bio-technological company. Then, the result of was used in comparison with some possible strains of bacteria on NCBI website. The results of traditional microbial identification experiments were also used to confirm the strain of bacteria. After the identification process, olive oil was used as the substrate for a series of batch experiments at different substrate concentrations. This research employed some microbial growth kinetic models to estimates the growth and decay of the bacteria obtained from the pure culture study. Two strains of bacteria was identified from the pure culture study were identified with the results on NCBI website and the results from traditional microbial identification experiments. Two species were identified, Thermus sp. and Microbacterium sp.. Pure Thermus sp. was used as the pure culture in batch experiment at the substrate concentrations in the range of 30 to 30000 mg/L TOC. The results indicated an increase in the substrate concentrations increased the µinitial when Thermus sp. was used as the microorgomisun. When the substrate concentration was higher than 3000 mg/L TOC, µinitial significant decreased because of the effect of substrate inhibition. When the concentrations of the substrate were 30, 300, 3000, and 30000 mg/L TOC, µinitial was 0.97, 3.89, 3.66, and 1.59 mg-cell/mg-cell-day, respectively. This study used Andrews’ inhibition equation to simulate the result of the batch experiments. The values of µmax 4.95 mg-cell/mg-cell-day, Ks 97 mg/L TOC, and Ki were 12000 mg/L TOC, respectively. The result showed Andrew’s equation was adaptable to estimate the kinetic parameters in this system.