The application of poly(lactic-co-glycolic acid) nanocomposites in bone regeneration

• Main Authors: Ming-Jui Kuo, 郭明瑞
• Other Authors: Shan-Hui Hsu
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/92457721608159835623

碩士 === 國立中興大學 === 化學工程學系所 === 96 === This research includes two different modification of poly-(lactide-glycolide) (PLGA). In the first part, PLGA films were mixed with different nano-calcium deficient hydroxyapatite (nCDHA) in a concentrative 50-1000 ppm. Cell proliferation and gene expression of ALP and OCN of bone marrow stromal cells (BMSC) were enchanced by the nonocomposite. Moreover, the osteoinductivity of PLGA-nCDHA composite kept ALP gene expression of the cells at 14 days. 50 ppm of nCDHA was the aptconcentration. In the second part, cellulose-binding domain arginine-glycine-aspartic acid (CBD-RGD) was successfully grafted onto the surface of PLGA by air plasma, confirmed by ESCA data. CBD-RGD also enhanced the adhesion and ALP gene expression of BMSC. It was concluded from this study that the use of air plasma was a powerful technique for modification of biomaterials, and nCDHA combined with CBD-RGD were conducive to bone differentiation of BMSC.