| Summary: | 碩士 === 國立中興大學 === 化學工程學系所 === 96 === This research includes two different modification of
poly-(lactide-glycolide) (PLGA). In the first part, PLGA films were
mixed with different nano-calcium deficient hydroxyapatite (nCDHA) in
a concentrative 50-1000 ppm. Cell proliferation and gene expression of
ALP and OCN of bone marrow stromal cells (BMSC) were enchanced by
the nonocomposite. Moreover, the osteoinductivity of PLGA-nCDHA
composite kept ALP gene expression of the cells at 14 days. 50 ppm of
nCDHA was the aptconcentration. In the second part, cellulose-binding
domain arginine-glycine-aspartic acid (CBD-RGD) was successfully
grafted onto the surface of PLGA by air plasma, confirmed by ESCA
data. CBD-RGD also enhanced the adhesion and ALP gene expression of
BMSC. It was concluded from this study that the use of air plasma was a
powerful technique for modification of biomaterials, and nCDHA
combined with CBD-RGD were conducive to bone differentiation of
BMSC.
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