Establishing Calibration System for Piston-type Micropipette and Measurement Uncertainty Evaluation

碩士 === 國立高雄應用科技大學 === 工業工程與管理系碩士班 === 96 === The concept of measurement uncertainty has already been the concerned by international organizations worldwide, as well as metrology researches and authorities of many countries for its extensive and practical applications. Especially in technology exchan...

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Bibliographic Details
Main Authors: Lien,Mien, 連綿
Other Authors: Gu-Hong, Lin
Format: Others
Language:zh-TW
Published: 2008
Online Access:http://ndltd.ncl.edu.tw/handle/59282712154772404424
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Summary:碩士 === 國立高雄應用科技大學 === 工業工程與管理系碩士班 === 96 === The concept of measurement uncertainty has already been the concerned by international organizations worldwide, as well as metrology researches and authorities of many countries for its extensive and practical applications. Especially in technology exchange or international trade, to gain mutual trust and recognition between two countries, it is necessary to present the uncertainty value in the measurement results. This study focused on the basic measuring instruments for medical science and laboratory science, and examined the measurement errors weights analysis according to the specifications of ISO8655-6. The basic principle is using micropipette to weigh the single-channel water, and multiplying the weight with the conversion coefficient (Z factor) of 1 and water density/air buoyancy, to obtain the volume of the channel. The establishment of the calibration system complies with the discussion of ISO/TR20461 technical specification on the uncertainty sources of piston-type micropipette in the calibration process, such as equipment, stability and traceability of standard component, proper environmental conditions, correct measuring method, and fully trained personnel. Based on the eight steps of evaluating uncertainty as suggested in the “Guide to the Expression of Uncertainty in Measurement” (ISO GUM), this study established an uncertainty evaluation model.