Preparation of Different Morphologies of ChondroitinSulfate-g-Poly(ε-Caprolactone) Nanoparticles As a DrugCarrier For Specific Cellular Uptake

• Main Authors: Hsiao-Chen Ni, 倪筱真
• Other Authors: Li-Fang Wang
• Format: Others
• Language: en_US
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/93851667752399042540

碩士 === 高雄醫學大學 === 醫藥暨應用化學研究所 === 96 === In this study, we introduced the double bonds onto a hydrophobic polycaprolactone (PCL) and a hydrophilic chondroitin sulfate (CS). The modified PCL was grafted onto the modified CS through a radical polymerization, which is abbreviated as PCL-CSMA. By controlling the composition between CS and PCL, the various morphologies of PCL-CSMA were obtained. Folic acid, a cancer mediated ligand, was grafted onto PCL-CSMA to improve the cellular uptake into tumor cells, which overexpress folate receptors. The PCL-CSMA copolymers with the different degrees of substitution were characterized by 1H nuclear magnetic resonance (1H-NMR), Fourier transform infrared (FTIR) and 1,9-dimethylmethylene blue (DMMB) assay. The sizes and morphologies of particles were studied by dynamic light scattering (DLS), Transmission electron microscope (TEM) and confocal laser scanning microscope (CLSM). The quantity of FAPEG conjugated on the particles was characterized by ultraviolet spectrophotometry. The quantity of FITC conjugated on the particles and the critical micelle concentration (CMC) were carried out with the use of fluorescence spectrophotometer. Zeta potential measurements of the particles before and after conjugation of FITC or FAPEG were also tested. The cellular uptake and distribution of the particles in KB cells and A549 cells were acquired with the use of flow cytometer and CLSM. A new type of amphiphilic PCL-CSMA copolymers was successfully prepared and potentially applied as drug carriers for anticancer agents in this study.