Induced differentiation of human embryonic stem cells into embryoid bodies and pancreatic islet-like cells, and comparison of their expression profiles of mRNAs and microRNAs.

• Main Authors: Bo-Zhi Chen, 陳泊志
• Other Authors: Steven Shoei-Lung Li
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/61727483443654816413

碩士 === 高雄醫學大學 === 醫學研究所 === 96 === Type 1 diabetes is an autoimmune destruction of pancreatic islet cell disease. It is an important development goal to find new alternative source of the islet cells to replace the damaged cells. Human embryonic stem (hES) cells are derived from the isolated inner cell mass of blastocysts, and they possess pluripotent potential and self-renewal capacity. The Embryoid Bodies (EBs) from hES cells were first formed by the suspension culture method, and the EBs were then treated with series of stimulus-induction to produce the pancreatic islet-like cells in vitro. The pancreatic islet-like cells were shown to express pancreatic specific markers of insulin, glucagons and somatostatin, and so on. The expression profiles of mRNAs and microRNAs from hES cells, EBs and pancreatic islet-like cells were further analyzed. MicroRNAs are endogenous small noncoding RNAs, and they negatively regulate the expression of protein-coding mRNAs. In this study, pancreatic islet-like cell were found to exhibit high expression of miR-186, 199a and 339, which possibly down-regulated the expression of undifferentiated markers such as LIN28. These islet-like cells were also found to have low expression of miR-19b, 20b, 221, 222, 92, 375, 200c, 302d, 367 and 372, which possibly resulted in the high expression of CNTN4, PIK3R1 and C11orf 9. Therefore, microRNAs are likely to play important regulatory roles in the cell differentiation and early embryonic development.