The study of immune function in alcohol consumption with various alcohol-metabolizing enzyme genotypes

• Main Authors: Sheng-Yi Chen, 陳勝義
• Other Authors: Liu-Yu Tsai
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/08257822384441457759

碩士 === 高雄醫學大學 === 生物醫學檢驗學研究所 === 96 === Objectives: To investigate the association of lymphocyte subpopulation, immunoglobulins, complements and cytokines levels in different alcohol consumption with various alcohol-metabolizing enzyme genotypes. Design and Methods: A questionnaire was used to distinguish the light, moderate and heavy alcohol drinker. A total of 83 light alcohol drinkers, 61 moderate alcohol drinkers and 70 heavy alcohol drinkers subjects were enrolled in this study. The activities of liver enzymes (AST, ALT, GGT), lymphocyte subpopulation (CD3, CD4, CD8, CD19, CD4/CD8 ratio), lipid (TG、Cholesterol), immunoglobulins (IgG, IgM, IgA), complements (C3, C4) and cytokines (IL-2R,IL-6,IL-8,IL-10,TNF-α) were analyzed. Genotypes of alcohol-metabolizing enzymes, including ADH2 and ALDH2 were analyzed with the single nucleotide polymorphism (SNP) analysis by real time PCR instrument. Results: There was a significant higher score of CAGE and AUDIT in the heavy alcohol drinkers than those of the light and moderate alcohol drinkers. For the liver enzymes, there was higher activity of AST and GGT in the heavy alcohol drinkers than those of the light and moderate alcohol drinkers. For the immune function tests, there were significantly higher levels in the heavy alcohol drinkers for the CD4, CD4/CD8 ratio and IL-6 than those of the the light alcohol drinkers and there were significantly lower levels in the heavy alcohol drinkers for the IgG and IgM than those of the light alcohol drinkers. However, there was no significant difference for the levels of C3 and C4 between the different alcohol drinkers. For the genotypes of alcohol-metabolizing enzymes, there were significantly differences in the allele, genotype, and phenotype of ALDH2 between the heavy alcohol drinkers and the light and moderate alcohol drinkers, but not for ADH2. To investigate whether the different combinations of ADH2 and ALDH2 could influence the immunity, we compared the levels of lymphocyte subpopulation, immunoglobulins, complements and cytokines in the subjects with different combinations of ADH2 and ALDH2 genotypes. The results showed that: ○1 there was a trend for the higher levels of IgG and IgA in subjects with the other combinations than the combinations of ADH2(*1/*1+*1/*2) and ALDH2(*1/*1) in the light alcohol drinkers . ○2 there was a trend for the higher levels of CD3 in subjects with the combinations of ADH2*2/*2 and ALDH2(*1/*2+*2/*2) than the combinations of ADH2(*1/*1+*1/*2) and ALDH2(*1/*1) in the heavy alcohol drinkers. ○3 there was a trend for the lower levels of CD19 in subjects with the combinations of ADH2(*2/*2) and ALDH2(*1/*1) than the combinations of ADH2(*1/*1+*1/*2) and ALDH2(*1/*1) in the heavy alcohol drinkers. Conclusions: Genetic variation of alcohol-metabolizing enzymes may play an important role on the levels of some lymphocyte subpopulation and immunoglobulins in heavy drinkers.