Chemical Constituents and Antitubercular Activity from Fatoua pilosa

• Main Authors: Chun-Ching Chiang, 姜純靜
• Other Authors: Ih-Sheng Chen
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/74320226382512584768

碩士 === 高雄醫學大學 === 天然藥物研究所 === 96 === Fatoua pilosa Gaud. (Moraceae) is a small perennial herb, which distributed in Taiwan, Philippine, Moluccas and New Guinea. In a series of studies on the active constituents of Formosan plants, the methanolic extract of the whole plant of this species showed antitubercular activity against Mycobacterium tuberculosis H37Rv in vitro. There are only three species of Fatoua in the world. The chemical constituents and bioactivities of Fatoua plants have never been conducted. The methanolic extract of whole plant of this species was partitioned into EtOAc and H2O-soluble fractions. Investigation of EtOAc-soluble fraction led to the isolation of six new coumarins: (+)-fatouain A (1), (-)-fatouain B (2), (+)-fatouain C (3), (-)-fatouain D (4), (+)-fatouain E (5), (-)-fatouain F (6) and four new bis-coumarins: (+)-fatouain G (7), (+)-fatouain H (8), fatouain I (9), fatouapilosin (10), along with eighteen known compounds, including three simple coumarins: umbelliferone (11), scopoletin (12), phellodenol-A (13), six furanocoumarins: psoralen (14), bergapten (15), S-(+)-marmesin (16), S-(+)-rutaretin methy1 ether (17), S-(+)-rutaretin (18), 2’,3’-dehydromarmesin (19), one pyranocoumarin: xanthyletin (20), three chaclones: isobavachalcone (21), kanzonol C (22), (E)-1-[2,4-dihydroxy-3-(3-methyl-2-butenyl)phenyl]-3-(2,2-di- methyl-8-hydroxy-2H-benzopyran-6-yl)-2-propen-1-one (23), one benzenoid: syringaldehyde (24), one quinone: α-tocopheryl quinone (25), one triterpenoid: lupeol (26), two steroids: a mixture of β-sitosterol (27) and stigamasterol (28). The structures of these compounds were elucidated by spectral analysis or by chemical derivation. Scopoletin (12), isobavachalcone (21) and (E)-1-[2,4-dihydroxy-3-(3-methyl- 2-butenyl)-phenyl]-3-(2,2-dimethyl-8-hydroxy-2H-benzopyran-6-yl)-2-propen-1- one (23) showed antituburcular acivity aganist Mycobacterium tuberculosis H37Rv in vitro with MIC values: 42.1, 17.6 and 30.0 μg/ mL, respectively.