| Summary: | 碩士 === 高雄醫學大學 === 公共衛生學研究所 === 96 === Abstract
Background and objective:
Lead is toxic to humans. It is known that exposure to lead for long-term harms the hematopoietic system. HFE mutation is associated with hereditary hemochromatosis that affects the iron absorption in the intestine, and develops iron overload. The objective of this study is to investigate whether the HFE genotype modifies the blood lead levels, affecting distributions of serum iron and the other red blood cell indices.
Material and methods:
121 lead workers and 117 non-exposure age-matched subjects enrolled in the study. We collected the following data from physical examination: blood lead levels, hemoglobin, hematocrit, serum iron, total iron binding caoacity and ferritin. And all subjects filled out questionnaires on the demographic information, medical history, alcohol consumption and smoking history. HFE genotyping for C282Y and H63D was using polymerase chain reaction and restriction fragment length polymorphism (PCR/RFLP).
Results:
The mean blood lead levels in lead workers was 19.75 μg/dL（SD = 14.70）and 2.86 μg/dL（SD = 1.85）for non-exposure subjects. The mean of serum iron in lead workers was 95.65 μg/dL（SD=41.13）,and 94.18 μg/dL（SD=40.86）for non-exposure subjects, which was not significant. Of 238 subjects, 221（92.9％）subjects were wild-type（CCHH） for HFE C282Y and H63D, and 17（7.1％）subjects were heterozygous for H63D mutation（CCHD）. All subjects in this study were free from C282Y mutation. In multiple linear regression analyses that adjusted for blood lead, HFE genotype（C282Y/H63D）, age, gender, smoking and alcohol consumption, serum ferritin for H63D heterozygous mutation was 122.715 ng/ml higher than H63D wild-type （P<0.05）. Using multiple linear regression to analyze the interaction relationship between blood lead and HFE genotype, the HFE genotype would be a modifier for the effect of lead to mean corpuscular volume（MCV）with adjustment of age, gender, smoking, and alcohol consumption. The effects of lead to MCV in the CCHH genotype was -0.116, while the CCHD genotype was 0.138.
Conclusions:
Our research found the significant modifying effect of HFE genotype on the lead affecting MCV. The HFE H63D heterozygous（CCHD）may be a protective factor. However, the toxic effects of lead on hematopoietic system need to study further in the other gene.
Key words: lead, HFE, hemochromatosis, ferritin, mean corpuscular volme
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