Development of oxidative stress damage model and protective effects of antioxidant agents in keratinocytes

• Main Authors: LEE,JUN-RU, 李俊儒
• Other Authors: LAN,CHENG-HANG
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/39900368707857144921

碩士 === 中華醫事科技大學 === 生物科技研究所 === 95 === The utilization of Radiometer to measure ultraviolet radiation in skin exposure assessment is limited since the lack of spectral irradiance and full wavelength scanning. An USB 4000 Miniature Fiber Optic Spectrometer was alternative used to measure the UVR emission and exposure dose from UV lamps. Following the measuring method, UVB induced oxidative stress in keratinocytes cell lines, xb-2 (mouse) and HaCaT (human), were establishes. This model was further applied to assess the protective effect of certain antioxidant agents. After UVB 60 mJ/cm2 exposure and then incubation 24 hr was found as the half efficiency does (ED50) in preliminary test. Both mouse and human keratinocyte cell lines showed the cellular ROS (reactive oxygen species), extracellular LDH (lactate dehydrogenase) increased, and in coincidence with the cellular survival (MTT test) decrease under the condition of ED50. The effects of antioxidant agents such as ferulic acid, feruloyl oligosaccharides, and Vitamin C showed protective capacity in this model. Based on the results from this study, the UVB exposure model could more accurately provides the information of spectrophotographies and UVB dosage. Moreover, the UV exposure model was evidenced to apply in evaluating the protective effect of antioxidant agents in keratinocytes. Key word：keratinocyte、ultraviolet radiation、antioxidant agents oxidative stress、Spectroradiometer system