Using sugar-composite chitosan particles for animal cell culture and virus production

• Main Authors: Wan-Ting Chen, 陳莞婷
• Other Authors: Ya-Ching Lin
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/hw5h7c

碩士 === 輔英科技大學 === 生物技術系碩士班 === 96 === Chitosan is a natural nontoxic biopolymer derived by the deacetylation of chitin. Chitosan has properties of biocompatibility, biodegradability,antibacterial activity and ease to adsorb the surface of cells. With these characteristics, it has attracted much attention as a new matrix for cell culture engineering. In this study, sugar-composite chitosan particles including glucose-chitosan particles, sucrose-chitosan particles and starchchitosan particles with varied concentrations of sugar were prepared for the culture of Vero and PK15 cells. Our results showed that among these particles, 30% starch-chitosan particles were the best carriers for Vero cells growth while 10% starch-chitosan particles were the best carriers for PK15 cells. Virus production in these two types of starch-chitosan particles, particular for enterovirus production, was better than that in culture flasks, suggesting promising application of the starch-chitosan particles to virus vaccine production.