| Summary: | 碩士 === 輔仁大學 === 營養科學系 === 96 === According to 〝Taiwan Elderly Nutrition and Health Survey〞 showed that the prevalence rates of vitamin B2 marginal deficient status for elderly people were around 30%. In order to study the immunological effects beyond different vitamin B2 status, we established 1% D-galactose (DG) induced aging in C57BL/6 male mice. Mice were divided to five groups including control group (subcutaneous (s.c.) PBS and received normal diet; NC), aging control (s.c. DG and received normal diet; AC), s.c. DG and combined with vitamin B2 deficiency diet (AD), s.c. DG and combined with vitamin B2 adequacy diet (AA), s.c. DG and combined with vitamin B2 marginal deficiency diet (AM). Besides, both AA and AM groups were pair-fed their diet in equally amounts refer to AD group. All groups receiving their special diet along with the period of study, on the 8th weeks of study, each mouse were s.c. injected with even PBS (NC group) or DG treatment (the other groups in spite of NA group) and after sixteen-week-feeding animals were sacrificed. There were no different on mice body weight, percentage of spleen weight relative to body weight, proliferation capacity of splenocytes, and percentage of splenocytes subpopulation among groups. Our data also indicated that the NK cell activity were increased, and the IgM and IgA titer in plasma were decreased as the vitamin B2 status towards deficiency. WBC contents were no difference in these groups, but higher counts were found in AC group as compared to NC group and paralleled with the decreasing as the vitamin B2 status reduced. In AD group, IFN-γ secretion of ConA stimulated splenocytes were significantly increased as compared to NC group. The levels of RAGE (receptor for advanced glycation end product) in plasma were increased with age, but in AD group it represented the lowest levels among groups. Vitamin B2 deficiency combined with DG treatment on C57BL/ 6 mice may induce some stress proteins expression which may compromise the following parameters, such as RAGE contents, leukocyte counts, levels of antibody secretion, enhanced NK cell activity and all contributed to modulating the immune response towards upregulating the innate immunity. Many studies reported that rat shown much higher growth rate than mice, which also explained that rat showed be more sensitive model to the change of vitamin B2 status. By the way, there were no documents reported that DG induced aging been applied on rat experimental model. So in the second part of this study, we compared 1 % or 5 % DG subcutaneous injection on SD rat, and fed with different vitamin B2 diets, than analytic its related immune function changes in the indicated groups: s.c. with PBS (NC), s.c. with 1 % DG combined with vitamin B2 adequacy diet (1A), s.c. with 5 % DG combined with vitamin B2 adequacy diet (5A), and s.c. with 5 % DG combined with vitamin B2 marginal diet (5M). Collected data show that these were no differences on the splenocyte proliferative response, the NK activity of splenocytes and phagocytosis activity of blood cells, among these three groups which treated with 1 % or 5 % DG (1A, 5A, 5M). The total leukocytes counts in 5M group were significantly lower than the 1A and 5A groups. In conclusion, DG induced aging seems not a proper study model for mimic the hyporesponsibility phenomenon occurs in natural aged individual. Interestly, our study also indicated that under the vitamin B2-deficiency status, related innate immune activity were upregulated and boosted.
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