Isoflavone extract as a cosmeceutical ingredient: evaluation of antiphotoaging activities in vitro and in vivo

• Main Authors: Tzu-Ju Lin, 林孜如
• Other Authors: Chi-Feng Hung
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/08531006734442409658

碩士 === 輔仁大學 === 基礎醫學研究所碩士班 === 96 === Ultraviolet (UV) irradiation causes pathological changes in the skin such as photoaging and skin cancer. Acute UV exposure on the skin causes erythema, inflammation, proliferation and differentiation. Twelve isoflavones are found in soybean and are present in four chemical forms including malonylglucosides, acetylglucosides, glucosides and aglycones. There are higher antioxidant acriviries of acetylglucosides and glucosides fractions than others. The aim of this study was to evaluate the antiphotoaging activities of the isoflavone extracts from soybean cake in vitro and in vivo experimental models. In our srudies, the extract of twelve isoflavones was defined as “ISO-1”. The faction of acetylglucosides and glucosides was defined as “Fraction 3”. In vitro study, human keratinocyte (HaCaT) cell was used to investigate the inhibitiory effects of p38, c-Jun N-terminal kinase (JNK) and extracellular signal regulated kinase (ERK) 1/2 phosphorylations. In vivo study, ICR-foxn/nu mice was irradiated by 150 mJ/cm2 of UVB for 7 days. The thickness of epidermis, catalase activity, cyclooxygenase-2 (COX-2) and proliferating cell nuclear antigen (PCNA) expression were used to evaluate the antioxidant and anti-inflammatory activities of isoflavone extracts. Our results show that the UVB-induced cell apoptosis and the phosphorylation of p38, JNK and ERK1/2 were apparently inhibited by isoflavone extracts in HaCaT keratinocytes. In vivo studies, we found that the thickness of epidermis, the expression of COX-2 and PCNA were increased after the UVB exposure. However, the catalase activity was decreased after UVB exposure. Treated with ISO-1 before UVB irradiation, the catalase activity was enhanced, the thickness of epidermis and the expression of COX-2 and PCNA were decreased. The similar results were also found in treated with fraction 3. However, treated with genistein before UVB irradiation did not enhanced the activity of catalase. Taken togather, our results demonstrate that there is higher antiphotoaging activity in ISO-1 than in genistein. Thus, the isoflavone extracts from soybean cake is suitable for developing as an antiphotoaging agents for skin care.