| Summary: | 碩士 === 大葉大學 === 生物產業科技學系 === 96 === The primary aim of this study was to determine the amount of green tea power, selenium yeast and chitosan which related to fat reduction, and formulate a new low-calorie, high-fiber, and good satiety weight-loss meal replacement, in order to match all the criteria of a commercial formula. We selected three different amounts of these three main ingredients as the test formula which were green tea powder (5g, 5.5g, 6g), selenium yeast (1g, 1.5g, 2g) and glucose powder (7g, 7.5g, 8g). A 3-factor, 2-level Response Surface Methodology (RSM) design was used to derive a second-order polynomial equation and to obtain the optimal viscosity and combination of the test formulas. The actual values of nutrient compositions were examined by different tests to make sure its correspondences to the designed values, Dietary Reference Intakes (DRIs). The storage time was assessed by measuring moisture content after it was laid aside for 180 days. The result showed that the most suitable combination of formula for green tea powder, selenium yeast and glucose powder was 5.4±0.07g, 1.3±0.2g, and 7.5±0.2g, respectively. The best viscosity value was 78 CPS (p < 0.05). From the result of 9-scale hedonic sensory evaluation, it showed that the milk-based formula had better taste than soy-based formula (p <0.05). The total energy was 136 kcal per pack and its energy distribution were 61% of total calories from glucose (21.7g), 19% from protein (4.7g), and 20% from fat (3.3g). The vitamin and the mineral contents conformed to the originally designed values which were proportional to the values of DRIs, Department of Health, Taiwan ROC. For the storage time, the average moisture contents of the test formulas were 8.6% and 8.8% before and after laid aside for 180 days. It did not have any deterioration or agglomerates. We formulated a pleasing palatable taste with suitable filling characteristics which contained adequate nutrients composition and may potentially become a tasteful and useful weight-loss meal-replacement commercial product in the future.
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