Studying on the Interaction of White Spot Syndrome Virus (WSSV) Structural Protein VP51A (ORF294) with Other Structural Proteins

• Main Authors: Yuan-Ting Lee, 李媛婷
• Other Authors: Yun-Shiang Chang
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/89151261482812844982

碩士 === 大葉大學 === 分子生物科技學系碩士班 === 96 === In this study, we characterized a novel white spot syndrome virus (WSSV) envelope protein, VP51A (WSSV-T1 ORF294), identified from a previous study. Membrane topology assay demonstrated that the VP51A 72 kDa protein is a type II transmembrane protein with a highly hydrophobic transmembrane domain on its N-terminal and a C-terminal that is exposed on the surface of virion. Co-immunoprecipitation, co-localization and yeast two-hybrid assays revealed that VP51A associated directly with some WSSV major structural protein, such as VP19,VP24 and VP26 and indirectly with VP28. Thus, these proteins may form a complex in the virion which may contribute to virus infection and to viral morphogenesis.