| Summary: | 碩士 === 朝陽科技大學 === 生物技術研究所 === 96 === Typhonium giganteum Engler (Araceae) is a perennial herb with an underground tuber. Dried tuber of T. giganteum, known as Bai fu zi, has been commonly used as traditional Chinese medicine for the treatment of headache, hemicrania and other syndromes. A standardized protocol for the establishment of callus culture using the root explants and mass- propagation of the plant is reported here. The optimum condition for callus induction is using a medium of full strength MS salts, 1.0 mg/l 2,4-D, 3% sucrose and 0.9% agar, and then growing in dark at 25±1°C for 8 weeks. And as for the maintaining of callus, medium containing full strength of MS salts, 1.0 mg/l 2,4-D and 0.2 mg/l BA were used. For the mass-propagation of the plant, the medium should contain full strength MS salts supplemented with 3% sucrose, 0.9% agar and 40 g/l banana pulp. To study the function of hemagglutinating activity of Bai fu zi, a number of cultured explants were collected. Crude extracts obtained after phosphate buffer saline (PBS) and 40-60% ammonium sulfate extraction followed by dialysis were used for the treatment of erythrocytes. The result showed a remarkable increasing in the hemagglutinating activity and the light even enhance such activity. The protocol established in this thesis can provide a new and promising source of T. giganteum for further biochemical study and medical applications.
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