The Anti-liver Fibrosis Effect of Nano-San Huang Xie Xin Tang

• Main Authors: Wan-ching Yang, 楊婉婧
• Other Authors: Walter H. Chang
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/01454851992875399087

碩士 === 中原大學 === 醫學工程研究所 === 96 === Rhei Ehizoma, Coptidus Rhizoma and Scutellariae Radix are three popular traditional Chinese herbs, commonly used in Chinese medicine prescription to treat liver disease. The extract prepared from the roots of Rhei Ehizoma, Coptidus Rhizoma and Scutellariae Radix (SHXXT) was demonstrated more excellent hepato-protective activity than the single herb whish used individually as indicated in this studies. The SHXXT are sorted three groups: water-boil, micro-powders and nano-powders. Liver fibrosis induced in male ICR mouse by oral administration with 5% chloroform twice a week for 8 weeks. At the same time, SHXXT (58 and 116 mg/BW) was administered intragastrically. The results was investigated that low dosage of Nano-SHXXT alleviated liver fibrosis induced by chloroform in mouse. Additionally, there was a remarkable improvement of CAT, SOD, GSH and GSH Px (GPx) activities. The results from in vitro studies showed MMP-2, TIMP-1, Bcl-2 mRNA expressions decreased. However, the increase Bax, Fas mRNA expression increased in hepatic stellate cells (HSCs) activated by TGFβ1. These results suggested that Nano-SHXXT could not only inhibited significantly the progression of hepatic fibrosis induced by chloroform, but inhibited the proliferation of activated hepatic stellate cells by TGF-β1.