System Integration Studies of a Radio Frequency Based Microscope Chamber
碩士 === 中原大學 === 醫學工程研究所 === 96 === The purpose of this study is to develop a radio frequency (RF) based microscope chamber system. The system includes a RF heating platform, a sensing subsystem, a control subsystem and an incubation chamber. The heating platform is driven by a class-E amplifier. A s...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2008
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| Online Access: | http://ndltd.ncl.edu.tw/handle/73085493498572622667 |
| Summary: | 碩士 === 中原大學 === 醫學工程研究所 === 96 === The purpose of this study is to develop a radio frequency (RF) based microscope chamber system. The system includes a RF heating platform, a sensing subsystem, a control subsystem and an incubation chamber. The heating platform is driven by a class-E amplifier. A spacer is designed in the heating platform for placing a RF transmission coil and a ring shape stainless steel plate. The sensing and the control subsystems are incorporated to control the chamber system in an ideal environment of 95% humidity, 37 oC and 5% CO2.
Optimization study results indicate that the thermal effect of the cell culture medium and the stainless steel plate can be achieved by driving the RF system at 200 KHz. A study was performed to evaluate the developed system including a 1-liter rectangular chamber by comparing to a standard incubator in the aspect of macrophage MTT performance. The study result indicates in a 24-hour incubation period about 89% of the cell survival rate in the RF based microscope chamber system is achievable.
In conclusion, the system integration of the RF based microscope chamber is accomplished in this course,. The performance of the system in terms of macrophage incubation is acceptable.
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