| Summary: | 碩士 === 中原大學 === 化學研究所 === 96 === Bisphenol A (BPA) and alkylphenols (APs) have become a public concern due to their massive use, ubiquitous occurrence, and persistency in the environment. BPA is mainly used as a monomer in the preparation of polycarbonate plastic and epoxy resins which are used in baby bottles, protective coatings on food containers, and so forth. The most commonly used APs are nonylphenol (NP) and octylphenol (OP) and are widely used as intermediates to produce surfactants and as stabilizers of ethylcellulose resin, oil-soluble phenol resins, and esters. After these compounds are manufactured into packaging materials and subsequently released into the environment, food and feed may contain some of these products. Bioassay studies have identified that BPA, OP, and NP elicited estrogenic potency and chronic toxicity. These compounds enter the food chain in several different ways. In humans, intake of these so- called environmental hormones is mainly via contaminated drinking water and foods.
The purpose of this study is to develop a method based on liquid chromatography tandem mass spectrometry (LC/MS/MS) for the simultaneous determination of BPA, NP, and OP in water from plastic containers. Concentration and clean up of samples are performed on 60 mg OASIS○R HLB solid extraction cartridges. The effects of mobile phases and additives on ionization are assessed. The recoveries range from 100.9 to 113.8% for BPA, 87.0 to 113.6% for NP and 88.4 to 117.6% for OP. The limits of quantitation (LOQ) of the method under multiple reaction monitoring (MRM) acquisition mode are 0.52, 0.10 and 0.18 ng/L for BPA, NP, and OP, respectively. BPA and NP can be detected in water samples and are at levels ranging from 2.23 to 69.30 μg/L for BPA, 0.14 to 1.84 μg/L for NP. Concentration of OP was below the LOQ.
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