Enhancement of Target-DNA Hybridization Efficiency by Pre-hybridization on Sequence Orientated Microarrayed Probes
碩士 === 中原大學 === 化學工程研究所 === 96 === The enhancement of hybridization efficiency of deoxyribose nucleic acid (DNA) targets by oigonucleotide pre-hybridization is studied on two sequence-inversed microarrayed probes. The sequences of both pre-hybridizing oligo and target DNA are designed to be fully co...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2008
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| Online Access: | http://ndltd.ncl.edu.tw/handle/17781946118706984023 |
| Summary: | 碩士 === 中原大學 === 化學工程研究所 === 96 === The enhancement of hybridization efficiency of deoxyribose nucleic acid (DNA) targets by oigonucleotide pre-hybridization is studied on two sequence-inversed microarrayed probes. The sequences of both pre-hybridizing oligo and target DNA are designed to be fully complementary to their shared DNA probe in a coaxial stacking configuration; i.e. they hybridize immediately next to each other along the continuous complement probe strand. The pre-hybridizing oligo and target DNA are differentiated by labeling with two distinct fluorescent dyes, and the cooperative effect on hybridization efficiency is investigated through the comparison between the stacking and individual hybridization configurations based on the detection signals of the labeling dyes.
The results show that the pre-hybridization of a DNA oligo enhances the subsequent hybridization efficiency of the target DNA coupling onto the same probe.
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