Ocular Hypotensive Affect Morphology Process in the Rat Model of Cyclodialysis

• Main Authors: Wei-Haw, 彭偉豪
• Other Authors: 廖克剛 副教授
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/54740197984138897055

碩士 === 中山醫學大學 === 醫學研究所 === 96 === Low-tension Glaucoma was a progressive optic neuropathy with characteristic optic disc changes, retinal ganglion cell loss and progressive visual field defects; Clinical case report publicized that traumatic cyclodialysis would reduce intraocular pressures(IOP>30%), maculopathy and visual function degeneration. The present study was aimed to observe the cyclodialysis rat of model morphology change with transmission and scanning electro-microscopic approaches. 30 adult male Wistar rats(8~12wks/300g) IOP was measured in awake using a calibrated Tonopen XL- tonometer. in normal eyes resulted in an average 27 +/- 5%mmHg. Unilateral cyclodialysis used 28-gauge hypodermic needle and orienting the spatula hugging the sclera to strip the iris root attachment away from the sclera. After surgery, The animal model eye ball was been hypotony as average an intraocular pressure (IOP) at or below 5 mmHg. (Table. 2) and experience side IOP trimmed down more than 30%(4mmHg+/- 5%) and measured IOP at the same time every day. The present results indicated that following 2 weeks unilateral cyclodialysis. We detected retina and optic nerve each three days in EM. After operation, the density of ganglion cell was lessen and axon were significantly less than in the control eyes.(control 63.1%+ 2.1%, exp 28.5%+3.4%, P<0.05) The optic nerve neuron to be abnormally enlarged and swollen , axon were characterized by accumulation of organelles, vacuole, cell shape was transformed. Based on these histological findings, unilateral cyclodialysis drop off IOP for some time, affect the retina cell death and optic nerve degeneration in morphology that could influence the visual function loss.