The Study of Correlation Between Osteopontin andHuman Osteoporosis

• Main Authors: Tasy- I, 江采宜
• Other Authors: 鄭雅文
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/05721102029367647821

碩士 === 中山醫學大學 === 醫學研究所 === 96 === Osteoporosis is of global concern especially due to its lack of clinical signs. In Taiwan, prevalence of vertebral fractures reached 18% in women and 12% in men older than 65 years, respectively. Among all, patients with hip fractures suffer with the most extent. Besides pain and disability from the disease, they also require long term medical care and constant assistance from others or auxiliary tools for activities; and these significantly affect their quality of life. Early diagnosis is considered the most effective method to prevent osteoporosis by far. Osteopontin (OPN) is a multifunctional protein secreted by bone cells. And osteopontin is considered to play an important role in the metabolism of bones, and is found to be associated with bone remodeling and strength of bones. Previous results from in vivo studies suggested that osteopontin may be related to onsets of bone resorption in osteoporosis. However, the association between osteopontin levels and osteoporosis in human remains unclear. Therefore, 122 postmenopausal women and 58 healthy women in their reproduction period were invited for study. Enzyme-link immunosorbent assay (ELISA) was used 4 to determine the serum osteopontin levels of each study subjects; bone density of each individual were also carefully detected. In our results, the mean serum osteopontin level was significantly different between experimental group and control group (14.8ng/ml vs. 7.8ng/ml, p<0.0001); and age is positively correlated with serum osteopontin levels (r=0.5963, P<0.0001). Moreover, the mean serum osteopontin level of individuals with a T-score less than -2.5 were found to be higher than those with a T-score larger than -1 SD(T-score <-2.5, OPN=15.2 ± 6.2 ng/ml; T-score>-1, OPN=13.2 ± 5.2 ng/ml). Body weight and bone density at the hip were also negatively correlated with the mean serum osteopontin level (Body weight, r=-0.2178, P=0.0188; and bone density at hip, r=-0.4313, P<0.0001). Subsequently, a multivariate regression model was used to detect any changes in bone density at the hip in relation to serum osteopontin level (β=-0.01/ng/ml). And individuals with higher serum mean osteopontin levels (≧10ng/ml) were found to have a 14.7-fold increase in risks of osteoporosis (95% CI=6.04-35.59) when compared to subjects with mean serum osteopontin levels less than 10ng/ml. We concluded that 5 postmenopausal women with higher serum osteopontin levels may experience elevated risks for osteoporosis than those having lower serum osteopontin levels. And the level of osteopontin in serum may be a promising marker for the diagnosis of osteoporosis.