| Summary: | 碩士 === 中山醫學大學 === 醫學分子毒理學研究所 === 96 === Serotonin Transporter (SERT) is located in the chromosome 17q 11.1-q12. The main biological function is responsible for reuptake of synaptic Serotonin (5HT) to neural terminals. Then, oxidation or restoration of Serotonin (5HT) is happened in neural cells to regulating the transmission of synaptic Serotonin (5HT). The popular antidepressant drug- Prozac (Fluoxetine) is the most famous Selective Serotonin Reuptake Inhibitor (SSRI). It is involved in the regulation of the mood、emotion、sleep and appetite in human being. And so, the traditional researches about Serotonin Transporters (SERTs) were focused on brain. But, our report was focused on cancer as a novel view. By using RT-PCR and Real time-PCR, we found that SERT expressed both in lung cancer cells (H1299、A549、Calu-1、CH27、H23、CL 1-1 and CL 1-5) and normal lung cells (MRC-5 and Beas-2B). In 18 NSCLC patients, expression level of SERT mRNA, analyzed by Real time-PCR, in lung tumor tissue was significantly lower than adjacent normal tissue. Moreover, we studied SERT protein expression in frozen specimens of lung-cancer tissue from 66 randomly selected patients who had undergone surgical resection of NSCLC and evaluated the association between the level of expression and survival. Poor prognosis is associated with the lower expression of SERT protein in lung tumor tissue. Our SERT protein expression is closely associated with the overall survival especially among the stage I disease and adenocarcinoma of the NSCLC patients. Therefore, the results of this study suggest that Serotonin Transporter (SERT) in lung is highly suspected of tumor suppressor gene.
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