Protein kinase C alpha expression in human endometrial and decidual tissues

• Main Authors: Tsung-Ho, 應宗和
• Other Authors: 劉哲育
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/25393521005875244341

博士 === 中山醫學大學 === 生化暨生物科技研究所 === 96 === In our previous studies, we had found that the decreased cytosolic PKC α was associated with the modulation of decidual cell growth in pseudopregnancy and pregnancy rats. The aim of this study was to determine whether the distribution of cytosolic PKCα in human decidual tissue was the same as in the pseudopregnancy and pregnancy rats. Eighteen specimens of normal endometrial tissues from patients post uterine surgery （uterine myoma）, and fifteen decidual tissues from legal elective abortion. All of these tissues were sent for determining the mRNA content of PKC isoforms by using RT-PCR, the result showed that PKC isoforms within these tissues with different amounts. We also observed the expression of PKCα by IHC analysis and western Blot. The results showed that the expression of PKCα was observed in both endometrial and decidual tissues by immunohistochemical staining. In decidual tissue PKCα mainly located in membrane area, while that in endometrial tissue revealed little staining. Moreover, western blot also confirmed that the PKCα expression in particulate fraction in decidual tissues were significantly higher than that in endometrial tissues（P<0.05）, but in cytosolic fraction there were no difference. We further measured the expression of matrix metalloproteinase （MMP-2 and MMP-9）by using zymography after endometrium and decidua cells were treated by PKC activator（TPA） and PKCα specific inhibitor （G06976）, the results showed that the expression of MMP-9 was significantly decreased paralled with the inhibition of PKCα but MMP-2 did not show the same inhibitory results. These results indicated MMP-9 expression may be influenced by activated PKCα, and suggested that PKCα may be involved in the human uterine decidualization.