Solanum nigrum water extract and polyphenol-rich extract suppress the migration and invasion in human hepatocarcinoma cell

• Main Authors: Yuan-Song, 施沅松
• Other Authors: Chau-Jong Wang
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/17902292369488158215

碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 96 === Metastasis is one of the most complicated and major pathologic processes responsible for poor prognosis of cancer patients. Studies of the molecular mechanisms for these processes are important for developing more effective antimetastatic strategies. Solanum nigrum L. is a common herb that grows wildly and abundantly in open fields. In this study, we have demonstrated that the water extract of Solanum nigrum L. (SNWE) and polyphenol-rich extract of Solanum nigrum L. (SNPE) suppressed TPA-induced HepG2 cell migration and invasion. To investigate the mechanisms of inhibited migration by SNWE and SNPE, we observed that SNWE and SNPE could reduce the PKCα-p38 pathway, Rho GTPase family and integrin signal pathway. Overexpression of constitutively active PKCα may restore the inactivation of p38 and the attenuation of cell migration by SNWE and SNPE. This study may have valuable implications for developing new therapies for clinical patient.