| Summary: | 碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 96 === Citrus fruit and peel contain many functional ingredients that are involved in anti-oxidation, anticancer, improvement of cardiovascular function and immunity. In order to develop citrus as healthy food, we prepared ethanolic extract from orange peel and orange seed and tested their potential in hypolipidemic effect that could be used in prevention of atherosclerosis and cardiovascular disease. The oxidative modification of low density lipoprotein plays a key role in the pathogenesis of atherosclerosis. Anti-oxidative reagents, which can effectively inhibit LDL oxidation, may prevent atherosclerosis via reducing early atherogenesis , and slowing down the progression to advance stages. We first defined the production rate, composition and anti-oxidative activity of orange peel extract (OPE)
Furthermore, we examined the effect of OPE on the serum lipids in the high cholesterol and high fat-fed hamsters. The results showed that all of OPE showed strong anti-oxidative activity as demonstrated by DPPH depletion assay and thiobarbituric acid-reactive substances (TBARS) assay. The data of weight gain, liver and adipose tissue observation of animal study revealed that OPE was able to improve the phenomena of fatty liver and decrease the weight of adipose tissue. Serum sample analysis of serum cholesterol, TG, and LDL-cholesterol showed that the supplement of OPE (2%、1%、0.5%) in the chow could effectively lower the hyperlipidemia caused by high-fat diet. Based on these findings, orange peel extract at the tested concentrations could efficiently lower the serum lipid levels and prevent the development of fatty liver caused by high fat food.
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