Study on the mechanism of the whitening effect of natural compounds fermented with Aspergillus oryzae

• Main Authors: Hui-min Tseng, 曾慧敏
• Other Authors: Ching -Gong Lin
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/23687872988285522175

碩士 === 嘉南藥理科技大學 === 化妝品科技研究所 === 96 === Kojic acid (5-hydoxy-4-pyran-4-one-2-methyl) is a tyrosinase inhibitor that it derived from various fungal species such as Aspergillus and Penicillium. However, some reports showed the carcinogenic potential and genotoxic risk of kojic acid. This study hyperpigmentation of the mechanism has attempted to find effect of whitening agent fermented from Aspergillus oryzae.The inhibition of tyrosinase activities and melanin formation in B16 melanoma cells of fermented or nonfermented by Aspergillus oryzae from traditional Chinese medical herbs (Rhizoma Zingiberi,Radix Astragali, Paeoniae Albiflorae Radix, Scutellatiae Radix,Ophiopogonis Tuber) and the purified isoflavones from fermented 5784''-TF(5,7,8,4''-Tetrahydroxyisoflavone),784''-TF(7,8,4''-Trihydroxyisoflavone ) were evaluated in this study. The experiments were evaluation mushroom tyrosinase activities of the extracts of Chinese medical herbs (Radix Astragali, Rhizoma Zingiberi, Ophiopogonis Tuber) and purified isoflavones by Aspergillus oryzae from fermented 5784''-TF, In vitro. Chinese medical herbs show that at 0.1mg/mL concentration was higher inhibition tyrosinase activity with data of 51.3%, 37.6%, 31.4%, and Daidzein, Genistein , 5784''-TF at 0.1mM concentration decrease 61.4%, 63.4%, 66.9%. The results show that Rhizoma Zingiberi, Radix Astragali fermented with Aspergillus oryzae significantly reduce the cellular melanin content and inhibitory tyrosinase activity in a dose-dependent reaction. While that at 0.1mg/mL concentraction, fermented Chinese medical herb extracts can cause different effect of inhibiting tyrosinase that Paeoniae Albiflorae Radix, Scutellatiae Radix, Ophiopogonis Tuber. All results show that 784''-TF, 5784''-TF can inhibit the melanin formation and secretion from B16 melanoma cells after fermentation treatment. Tyrosinase zymography was use to make sure Rhizoma Zingiberi, Radix Astragali fermented from Aspergillus oryzae reduce B16 cellular tyrosinase activity expression. In this study, we use a chromatography with CIE L*a*b* colour system to observe the whitening efficacy when apply on the human skin. The results show that ten voluteers all apparently improving, then confirmed the significantly reduce melanogenesis after the cream treatment. Our conclusion show that Chinese medical herbs (Rhizoma Zingiberi, Radix Astragali, Paeoniae Albiflorae Radix, Scutellatiae Radix, Ophiopogonis Tuber) and purified isoflavones by Aspergillus oryzae from fermented products, significantly reduce the cellular melanin and inhibit tyrosinase activity in B16 melanoma cell.This study guess the biotransformation of secretion with Chinese medical herbs by enzymes and catalyzed as effective skin-whitening angents from fermented with Aspergillus oryzae.Therefore,there will developed effective skin-whitening angents for cosmetic markets in future.