| Summary: | 碩士 === 嘉南藥理科技大學 === 生物科技研究所 === 96 === Tripterygium wilfordii Hook F. (TWHF) is a celastraceae vine climber, and is a Chinese herb used to treat rheumatoid arthritis and systemic lupus erythematosus, and functions as an anti-inflammatory, immunosuppressor and antitumor. The focus of this study is to establish the technique in tissue culture of TWHF, and attempt to get the undifferentiated calli and induce shoots, roots, and even a whole plant. Stems and leaves of TWHF were cultivated in MSB1D0.2 (MS medium with 1 mg/l BAP and 0.2 mg/l 2,4-D) and MT2B1D0.5 (MS medium with 1 mg/l BAP and 0.5 mg/l 2,4-D and coconut water) media to induce calli. These were then transplanted into suspension culture at different rotation rates and cultivated at different temperatures, light and media conditions. Recent results showed the stem and leaf explants induced calli better at MT2B1D0.5 medium with the ratio of 81 % and 79 % respectively. Calli grew better at 100 rpm, 28 ℃ and dark circumstances with the increased ratio of weight of 85 %, 81 % and 73 % respectively. White calli turned green and grew better at 700 lux with an increased ratio of weight of 92 %. The medium with 0.1 mg/l NAA induced roots with the ratio of 60 %, the root number varied from 2 to 14, and the most length of root was 1.75 cm. 93 % of buds were induced from nodes, the most length of bud was 1.32 cm. 87 % of buds developed a plantlet in 1/2 MS medium with 0.1 mg/l NAA, the root number varied from 8 to 15, and the most length of root was 2.13 cm.
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