| Summary: | 碩士 === 中國醫藥大學 === 藥學系 === 96 === There are two kinds of herbs belongs to Molluginaceae Mollugo in Taiwan at present, one is M. stricta, and the other is M. verticillata. Both of them are annual herbs, mainly distributes in the tropics and subtropics area of European, Asia and Africa, and the Pacific Ocean various archipelagoes. In Taiwan, They could be found in the flats and low elevation mountainous area. We can find easily in the uncultivated land, the cultivated land and dry farm.
The purpose of this research is mainly to find the differences between M. stricta and M. verticillata by the appearance (fruit, seed, pollen) and histological anatomy.
However, after we duplicated ITS (Internal Transcribed Spacer) region that extracted from plant by the technology of PCR (Polymerase chain reaction). Since ITS region is different in each species even they are the same genus. It is usually used in identification of the prior plants. By sequencing, we found the ITS1 length and ITS2 length of M. stricta is 205 bp and 221 bp, the G + C contents of ITS1 and ITS2 regions are 53.2 % and 53.4 % respectively. ITS1 and ITS2 length of M. verticillata is 186 bp and 214 bp, the G + C contents of ITS1 and ITS2 regions are 66.7 % and 67.8 % respectively.
We have set up a quantitative method by HPLC to determine the quantity of Vitexin and Eriodictyol in herbs. This method have validated and showed high sensitivity, reproducibility, precision and recovery rate. The result showed that the Vitexin and Eriodictol content of M. stricta are 0.014 ％ and 0.383 ％ respectively.
In this study, we have found the best method for identify M. stricta and M. verticillata. And then, we investigate the effects of M. stricta on the carbon tetrachloride (CCl4)-induced experimental hepatic damage in rats and mice.
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