Analysis of Organic Solvent Mixtures in Urine Using Hollow Fiber Headspace Liquid-phase Microextraction and Gas Chromatography with Flame Ionization Detector

• Main Authors: Hong Chung Wei, 洪崇惟
• Other Authors: 許憲呈
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/marf2f

碩士 === 長榮大學 === 職業安全與衛生學系碩士班 === 96 === Multiple organic solvents, such as benzene, toluene, xylene, n-hexane, n-heptane, n-octane and methylcyclohexane, were used in an adhesive of factory. Workers exposure to these different kinds of solvents in the workplace. These substances will be translated into different kinds of metabolites. Therefore, biological monitoring of workers exposed to organic solvents is important step toward the evaluation of the toxicological effect of chemical solvents. In this study, a hollow fiber headspace liquid phase microextraction（HF-HS-LPME） and gas chromatography-flame ionization detector（GC-FID） method for the analysis of multiple organic solvent mixtures in urine was described. Extraction parameters such as extraction solvent selection, stirring rate, salt addition, extraction time, extraction temperature and pH were found to have significant influence on extraction efficiency. The optimized conditions were 2.0 l 1-octanol extraction solvent, 10 min extraction time, 40℃, 500 rpm stirring rate, pH 7.0 and without NaCl addition. Urine samples were obtained from exposed workers in the adhesive factory and were successfully analyzed using the proposed method. The results showed that the amounts of organic solvent mixtures in the end of shift urine samples were higher than those of in the pre-shift urine samples. This optimized method is suitable for the analysis of multiple organic solvent mixtures in urine samples.