Development of Co-immobilization system for ethanol production from starch by Aspergillus awamori, Rhizopus japonicus, and Zymomonas mobilis.

• Main Authors: Ming Te Chiu, 邱銘得
• Other Authors: Y. K. Liu
• Format: Others
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/21307114373567333492

碩士 === 長庚大學 === 化工與材料工程研究所 === 96 === The aim of the study was to produce ethanol from potato starch by co-immobilized Zymomonas mobilis, Aspergillus awamori and Rhizopus japonicus using PU carrier for the development of second biomass energy. Compare the ethanol production in the traditional methods with yeast and two-stage process and the microorganisms co-immobilize culture system, the immobilization system had better output than non-immobilization system. In other words, from the first co-suspension culture system, the two-stage process consisting of saccharification using co-immobilized Aspergillus awamori & Rhizopus japonicus(A-R system) and ethanol fermentation using immobilized Zymomonas mobilis, to co-immobilized Zymomonas mobilis, Aspergillus awamori and Rhizopus japonicus (A-R-Z system) in a single reactor, the ethanol production was increased from 2.423 g/l (Co-suspension culture system after 48hr.), 17.71 g/l (two-stage immobilization culture system after 84hr.), to 28.33 g/l (Co-cultured in a single reactor). In fed-batch fermentation, the ethanol can reach max. concentration 95.97 g/l (under the repeated 3 batches culture mode of operation after 72 hr.). Besides, the effect that increase raw starch concentration was raised from 6% to 12% (wt.), the ethanol can reaches max. concentration 60.18 g/l after 51 hr. The result proved the performance not only can enhance ethanol yield, but also has great benefit for efficiency advancement and less equipment.