Study on Thrombolysis and Inhibition of Rat Glioma C6 Cell of Nano-Fe3O4/PAn Composites

• Main Authors: Jui Chia Lai, 賴銳嘉
• Other Authors: Y. H. Mu
• Format: Others
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/11836166477959659405

碩士 === 長庚大學 === 化工與材料工程研究所 === 96 === The purpose of this study was to synthesize nano magnetic Fe3O4/polyanniline (Fe3O4/PAn) particles by co-precipitation and rapid cooling process to control the particle size. The droplet of polyanniline/dimethylsulfoxide (PAn/DMSO) solution was added to nano-Fe3O4 particles suspension by ultrasonic stirring and the nano-Fe3O4 particless were encapsulated with a layer of PAn. The obtained nano-Fe3O4/PAn composites were used as drug carriers for thrombolysis, which could inhibit the growth of rat glioma C6 cell. The structures of successfully synthesized nano-Fe3O4/PAn composites were analyzed by X-ray Diffractometer (XRD) and Fourier Transformed Infrared Spectroscopy (FT-IR). As measured by Superconducting Quantum Interference Device Magnetometer (SQUID), the saturation magnetization of nano-Fe3O4/PAn composites was decreased from 66.2 emu/g to 36.0 emu/g, because the magnetic interaction between Fe3O4 particles was reduced by PAn. According to Field Emission Scanning Electron Microscopy (FE-SEM ) and Transmission Electron Microscopy (TEM) analyses, the diameter of nano-Fe3O4/PAn composites was about 50~70 nm, which showed less aggregation of Fe3O4 particles due to the particle surfaces covered with PAn layer. From thrombus dissolution experiment, the dissolution effect of thrombus was increased 7~9% due to the enhanced magnetic field of nano-Fe3O4/PAn particles. After grafting with nano-Fe3O4/PAn/GA composite, it was measured that the grafting ratio of 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) was 56% and the drug release percentages was 94.5%. After in vitro reacted with rat glioma C6 cells (C6 cells) for 12 hours, the cell viability was decreased to 51.4%.