Analysis and occurence of pathogenic microorganisms in Taiwan surface watershed and control strategies of waterborne disease after seismic disaster

• Main Authors: Jung-Sheng Chen, 陳榮盛
• Other Authors: Bing-Mu Hsu
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/08848519755870985420

碩士 === 國立中正大學 === 地震研究所暨應用地球物理研究所 === 96 === Salmonella, Shigella and some opportunistic pathogens (Aeromonas, Citrobacter, Yersinia, Klebsiell, Serratia and Arcobacter, etc.) have emerged as waterborne pathogens of concern. The aim of this study was to examine the occurrence of bacterial pathogens and to identify the species of bacterial pathogens by biomolecular techniques in Taiwan surface watershed. We also evaluate different methods for detecting pathogens and investigated the relations between the pathogens and water quality parameters.The frequency of occurrence for Aeromonas spp. were 100% and 95.6% while analyzing by cultural method and direct extraction method, respectively. Five genotypes of Aeromonas spp. can be identified while combined multiples PCR and t-PCR. The frequency of occurrence for pathogenic Aeromonas were 78% and 58% in two selected watershed.The occurrence frequency was 28.9% for Shigella spp. for all collected water samples. The presence of shigella spp. and water quality parameters do not exhibit significant correlation. After sequencing analysis, eleven isolates were identified as S. flexneri 2a (68.8%) and five isolate was identified as S. flexneri 5 (31.2%) .The occurrence frequency was 42.2% for salmonella spp. for all collected water samples. High significant correlation was found between the presence of Salmonella and water turbidity. Eighteen positive water samples were identified as S. typhimurium but one sample was identified as S. enterica.The species of opportunistic pathogens isolated and identified from 8 surface watershed include Citrobacter freundii., Yersinia pseudotuberculosis , Yersinia enterocolitica, Klebsiell oxytoca., Serratia plymuthica and Arcobacter cryaerophilus.The presence of pathogens in Taiwan drinking watershed indicates the possibility of waterborne transmission in Taiwan if water is not adequately treated.