The Surface Enhanced Fluorescence of Fluorophores in the Phospolipid Bilyer on Roughness Silver film
碩士 === 國立中正大學 === 化學所 === 96 === The goal of this thesis is to design a membrane-associated biomolecule sensor by combing the lipid membrane which can select membrane affiliated biomolecules and the surface enhanced fluorescence (SEF) technique. SEF can improve the fluorescence detection sensitivity...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2007
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| Online Access: | http://ndltd.ncl.edu.tw/handle/13688569709217482122 |
| Summary: | 碩士 === 國立中正大學 === 化學所 === 96 === The goal of this thesis is to design a membrane-associated biomolecule sensor by combing the lipid membrane which can select membrane affiliated biomolecules and the surface enhanced fluorescence (SEF) technique. SEF can improve the fluorescence detection sensitivity.
We used the silver-mirror reaction to coat the silver nano-particles on a glass. Firstly﹐we proved the silver particles that we made are nano-scaled and exhibit the surface enhanced electric field. Then﹐we used the sol-gel reaction to coat a layer of silicon oxide on the top of the silver particles. Because of the silicon oxide﹐the fluorescence molecule did not touch the nano-silver particle directly and the fluorescence was not quenched by the silver nano-particle. We applied phospholipid bilyers containing fluorescence molecules to the substrate that has no enhanced fluorescence effect and to the substrate that has enhanced fluorescence effect to compare the fluorescence signals. We found that both the thickness of the silicon oxide and the absorbance of the silver particle on glass affect the surface enhanced fluorescence. An optimal condition is achieved when the thickness of the silicon oxide is 5nm and the absorbance of the silver particle on glass at 432nm is 0.40. In such case﹐the fluorescence enhancment factor is about 7~8.
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