Biomimicked poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) membranes for promoting the 3D morphological developments of C3A cells

• Main Authors: Yin-Hsing Chang, 張瑛鑫
• Other Authors: Yi-Ming Sun
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/37354668904833040208

碩士 === 元智大學 === 化學工程與材料科學學系 === 95 === The objective of this study is to mimic the natural cell environment on polymer surface for C3A cell culture. In this study, poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) and fibronectin were constructed as biofunctional membranes. AFM data and SEM image showed that the surface roughness of the PHBHHx membrane decreased after ozone treatment, but did not change significantly after grafting with acrylic acid (AA). The root-mean-square surface roughness of the unmodified PHBHHx surface was about 330 ± 22 nm. When the PHBHHx surface was treated with ozone for 60 min, the surface roughness decreased to 137 ± 3 nm. After grafting with AA, the surface roughness was 143 ± 4 nm. The alamarBlue test showed that C3A cells exhibited similar metabolic activity on those membranes and the MTT test revealed that cells exhibited good growth activity up to 9th day. The secretion of albumin from C3A cells significantly increased when they cultured on the AA grafted and fibronectin immobilized PHBHHx membranes. The morphology of C3A cells was spheroid-like on the AA grafted and fibronectin immobilized membranes and the three- dimensional (3D) morphology might promote the functions of C3A cells. In contrast, the morphology of C3A cells on unmodified membranes was two-dimensional (2D) monolayer.